![]() In traditional PCR, a single sample offers only a single measurement, but in Droplet Digital PCR, the sample is partitioned into 20,000 nanoliter-sized droplets. Sample partitioning is the key to droplet digital PCR. This technique has a smaller sample requirement than other commercially available digital PCR systems, reducing cost and preserving precious samples. The Droplet Digital PCR System partitions nucleic acid samples into thousands of nanoliter-sized droplets, and PCR amplification is carried out within each droplet. The massive sample partitioning is a key aspect of the ddPCR technique. The droplets serve essentially the same function as individual test tubes or wells in a plate in which the PCR reaction takes place, albeit in a much smaller format. Droplets are formed in a water-oil emulsion to form the partitions that separate the template DNA molecules. Droplet Digital PCR technology is a digital PCR method utilizing a water-oil emulsion droplet system. ![]()
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